As successor of the PULSE program family, PATCHMASTER now offers additional features meeting the highest standards of modern electrophysiology. This new software, running on PC-based and Macintosh computers, harbors all comfortable functions of PULSE, but offers a variety of novel procedures aimed to make electrophysiological research even more versatile and efficient.
With PATCHMASTER experimental design, performance, and analysis become much more flexible giving rise to a high degree of automation and providing access to experimental protocols that were thus far unattainable with commercial software.
More information about thisJust three clicks to whole-cell configuration. When used with HEKA's fully computer controlled amplifiers PATCHMASTER comes with pre-defined protocols which automate the major steps needed to establish the desired recording configuration. This gives the user room to draw his full attention to tasks such as approaching the cell and forming a giga-seal whereas adjustments on the amplifier and in the software are automated.
The Amplifier and I/O Control dialog windows gives you direct access to the hardware. Other devices like perfusion systems can also be controlled by the software in an exactly timed manner. PATCHMASTER's Pulse Generator offers extremely versatile stimulation, acquisition, including online data processing with up to 32 channels. Individual stimulation/acquisition sequences can be directly linked with appropriate analysis methods. The Online Analysis allows to pre-define different analysis methods that can plot the results to the notebook and display them in up to 12 individual graphs. Experiment control, automation and standardization can be achieved with the powerful programmable Protocol Editor which allows to interconnect different tasks in PATCHMASTER in a convenient way.
PATCHMASTER's data file can be directly read by FITMASTER which can be used for more detailed analysis of the data. Analysis results will be added to the data tree assuring a direct link between raw data and derived results. Export functions allow to transfer the data to other programs such as IGOR, MatLab, or save the date and results as ASCII files.
Features:
Full software control of iTEV 90, EPC 10, EPC 9, and EPC 800 amplifiers
Supports clusters of EPC 10 amplifies and EPS 16 probe selectors
All other amplifiers (e.g. EPC 8, EPC 7 Plus, or other brand) are supported in combination with an ITC-18, ITC-16, LIH 1600, or LIH 8+8 interface
Runs on Windows 7, 8, 10 and on Mac OS X ≥10.6 / ≤10.14 (requires a free USB port for the dongle)
Just three clicks to a whole-cell configuration
Pulsed and continuous acquisition modes
Automated filter adjustment to obey Nyquist theorem
Stimulation with recorded AP waveforms
Fast switching between current and voltage clamp
Chart display
Reference trace/series subtraction
Online data averaging
p/n leak subtraction
Flexible analysis with up to 12 online graphs
Versatile programming interface for task automation
Includes the following extensions:
Software Lock-In Amplifier
Photometry Extension
Solution Database
All software updates for our PATCHMASTER software are free of charge.
We provide a variety of training materials to accelerate your success with PATCHMASTER:
We offer short videos explaining the versatile functionality of PATCHMASTER on our HEKA YouTube channel.
The PATCHMASTER 101 webinar focuses on the needs of PATCHMASTER beginners and the platform is demonstrated in real time, allowing attendees to duplicate steps at their system. Live sessions are held periodically throughout the year, a recording is available on demand at any time.
The PATCHMASTER Tutorial library contains a wealth of information (PDFs) related to various aspects of the software. With information ranging from setting up a first experiment to navigating more complicated approaches, there is guidance available for every level of PATCHMASTER user.
Check out our list of the numerous PATCHMASTER configurations available to help demonstrate the versatility of this powerful software.
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